Mature miRNA in expression vector?
Dear All, I am planning to clone mature miRNA in expression vector.So,my question is ,can clone mature miRNA into the expression? or it should be in precursor form? and why? Many Thanks
View ArticlemiRNA Identification
Hi, I am completely novice to miRNA field. Just started reading and getting information. Can you expert please answer my questions? How do you make sure that miRNA exists in your treatment samples?...
View ArticleTarget gene cloning
How are you ladies and gentlemen, I am quite a newbie in this field of miRNA and I have a question. I am trying to clone a plant miRNA and its target gene and further overexpress them in a plant...
View ArticlemiRNA cloning
Good day ladies and gentlemen, I would like to ask something about plant pre-miRNA cloning. If I already have the sequence of the pre-miRNA, is there a rule for the 100bp 5' and 3' flanking region?...
View Articlemake siRNA-FITC labeled more concentrated
Dear all, I want to transfect my cells with siRNA-FITC labeled using electroporation. Results were not good comapny recommend to use conc equivalent to 100-250uM. my siRNA already reconstituted in...
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negative control miRNA
Hello everybody, Can anybody suggest a good negative control miRNA sequence for a mouse cell line ? Thanks, Dave
View ArticleWhere can I find 100-200 unique primer sequences for preparation of array
Hi everyone, I plan to synthesize about 5000 oligonucleotides on a chip and then use it as a template to prepare an array (100 pools/50 oligos each). The idea is to flank 50 oligos from one pool with a...
View Articlelizard microRNA and human lncRNA
I have two questions, first, how does the human long non coding RNA (lnc-RNA) changes the gene expression epigenetic marks and how can it target a particular cell for delivery? To being with, lizard...
View Articlesalamander wound regeneration processes compare to human model
So here is my speculation of what happens when a salamander attempts to regenerate the lost limb. First, the missing limb wound place sends a signal requesting macrophages for stem cell generation...
View ArticleCan a siRNA derived from hairpin structure just like miRNA?
Hi everyone, nice to meet everybody in here, I got a simple question. As far as I know, miRNA is derived from the long ssRNA sequence which can form a hairpin. And by introducing a specifically...
View ArticleWe need a way to see microRNA and DNA in vitro for reverse aging
Imagine being able to observe microRNA and DNA action in vitro, that, with the help of computer visualization, could help decode every mechanics in the body and eventually a way to reverse aging. From...
View ArticleUsing miR-PREFeR: is this a microRNA?
Sorry for this silly question, but I just wanted to get this right. I am fairly new to miRNA research and I've been using several miRNA-detection tools lately, e.g. mirDeep2* and miR-PREFeR. The...
View ArticleAll the same family miRNAs have the same MRE always?
Hello! I am new in the miRNAs topic and I have a question about it ... can someone help me? In my investigation I identified miRNAs that target Foxl2 at ovarian follicular activation time and found...
View ArticleWatch miRNA transcription start to happen in an embryo
Watching dynamics of RNA expression - paper describing a visualization technique. Movie 1 from supplemental information: in a zebrafish embryo, DNA is stained with red fluorescence and...
View Articlespike-in control
Hello, Im new to microRNA. As all qPCR reactions have to be normalised, can someone help me understand what is spike-in control for miRNA normalization and how it work? When do you we use...
View Articleurinary exosomal miRNA
Hi, I plan to study miRNA profile in urine. Is it best to study: (i) combine urinary circulating miRNA + exosomal miRNA (ii) study urinary circulating miRNA separately from exosomal miRNA. when we...
View ArticleHow to retrieve miRNA sequences from Ensemble using their coordinates ?
Dear all, I am facing a technical issue in miRNA. I want to retrieve the sequences of 500 duck miRNAs from ENSEMBLE database using their coordinates. For example I have a miRNA genomic location named...
View ArticleHow to retrieve miRNA sequences from Ensemble using their coordinates ?
Dear all, I am facing a technical issue in miRNA. I want to retrieve the sequences of 500 duck miRNAs from ENSEMBLE database using their coordinates. For example I have a miRNA genomic location named...
View ArticleHelp in gene editing
I am looking for human neuronal cell lines that can be engineered for various mutations using Crispr/Cas9 technologies.Can someone suggest what are the available neurological cell lines that can be...
View ArticlemiRNA quality and integrity
HI, Im new to miRNA and arrays. I have to perform an miRNA array and as these are very expensive, I want to check the quality of my extracted miRNA from serum prior to using arrays. 1. the...
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